Stephen Bustin
Stephen Bustin
Professor of Molecular Medicine, Anglia Ruskin University
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Cited by
Cited by
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
SA Bustin, V Benes, JA Garson, J Hellemans, J Huggett, M Kubista, ...
Clinical chemistry 55 (4), 611-622, 2009
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
SA Bustin
Journal of molecular endocrinology 25 (2), 169-193, 2000
INVITED REVIEW Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
SA Bustin
Journal of molecular endocrinology 29, 23-39, 2002
Quantification of mRNA using real-time RT-PCR
T Nolan, RE Hands, SA Bustin
Nature protocols 1 (3), 1559-1582, 2006
Real-time RT-PCR normalisation; strategies and considerations
J Huggett, K Dheda, S Bustin, A Zumla
Genes & Immunity 6 (4), 279-284, 2005
The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans
S Gnanapavan, B Kola, SA Bustin, DG Morris, P McGee, P Fairclough, ...
The journal of clinical endocrinology & metabolism 87 (6), 2988-2991, 2002
Quantitative real-time RT-PCR–a perspective
SA Bustin, V Benes, T Nolan, MW Pfaffl
Journal of molecular endocrinology 34 (3), 597-601, 2005
Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
SA Bustin, T Nolan
Journal of biomolecular techniques: JBT 15 (3), 155, 2004
Validation of housekeeping genes for normalizing RNA expression in real-time PCR
K Dheda, JF Huggett, SA Bustin, MA Johnson, G Rook, A Zumla
Biotechniques 37 (1), 112-119, 2004
Parameters for successful PCR primer design
SA Bustin, R Mueller, T Nolan
Quantitative Real-Time PCR: Methods and Protocols, 5-22, 2020
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
JF Huggett, CA Foy, V Benes, K Emslie, JA Garson, R Haynes, ...
Clinical chemistry 59 (6), 892-902, 2013
Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
C Tricarico, P Pinzani, S Bianchi, M Paglierani, V Distante, M Pazzagli, ...
Analytical biochemistry 309 (2), 293-300, 2002
The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
K Dheda, JF Huggett, JS Chang, LU Kim, SA Bustin, MA Johnson, ...
Analytical biochemistry 344 (1), 141-143, 2005
MIQE precis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments
SA Bustin, JF Beaulieu, J Huggett, R Jaggi, FSB Kibenge, PA Olsvik, ...
BMC molecular biology 11 (1), 1-5, 2010
Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis
SA Bustin, R Mueller
Clinical Science 109 (4), 365-379, 2005
The expression of the growth hormone secretagogue receptor ligand ghrelin in normal and abnormal human pituitary and other neuroendocrine tumors
M Korbonits, SA Bustin, M Kojima, S Jordan, EF Adams, DG Lowe, ...
The journal of clinical endocrinology & metabolism 86 (2), 881-887, 2001
AZ of quantitative PCR
SA Bustin
International University Line, 2004
Why the need for qPCR publication guidelines?—The case for MIQE
SA Bustin
Methods 50 (4), 217-226, 2010
Vitamin D receptor (VDR) mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity, and VDR genotype in Bangladeshi Asians
BW Ogunkolade, BJ Boucher, JM Prahl, SA Bustin, JM Burrin, K Noonan, ...
Diabetes 51 (7), 2294-2300, 2002
SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations
T Nolan, RE Hands, W Ogunkolade, SA Bustin
Analytical biochemistry 351 (2), 308-310, 2006
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