Split intein mediated ultra‐rapid purification of tagless protein (SIRP) D Guan, M Ramirez, Z Chen Biotechnology and bioengineering 110 (9), 2471-2481, 2013 | 77 | 2013 |
Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification M Ramirez, N Valdes, D Guan, Z Chen Protein Engineering, Design & Selection 26 (3), 215-223, 2013 | 70 | 2013 |
Bringing biocatalytic deuteration into the toolbox of asymmetric isotopic labelling techniques JS Rowbotham, MA Ramirez, O Lenz, HA Reeve, KA Vincent Nature communications 11 (1), 1454, 2020 | 64 | 2020 |
Intein-triggered artificial protein hydrogels that support the immobilization of bioactive proteins M Ramirez, D Guan, V Ugaz, Z Chen Journal of the American Chemical Society 135 (14), 5290-5293, 2013 | 57 | 2013 |
Two-component protein hydrogels assembled using an engineered disulfide-forming protein–ligand pair D Guan, M Ramirez, L Shao, D Jacobsen, I Barrera, J Lutkenhaus, ... Biomacromolecules 14 (8), 2909-2916, 2013 | 38 | 2013 |
Rapid, heterogeneous biocatalytic hydrogenation and deuteration in a continuous flow reactor LA Thompson, JS Rowbotham, JH Nicholson, MA Ramirez, C Zor, ... ChemCatChem 12 (15), 3913-3918, 2020 | 17 | 2020 |
E. coli Nickel‐Iron Hydrogenase 1 Catalyses Non‐native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme Ene‐reductases** S Joseph Srinivasan, SE Cleary, MA Ramirez, HA Reeve, CE Paul, ... Angewandte Chemie 133 (25), 13943-13947, 2021 | 8 | 2021 |
Intein mediated purification of protein Z Chen, MA Ramirez, D Guan US Patent 10,087,213, 2018 | 6 | 2018 |
Synthesis of an intein-mediated artificial protein hydrogel MA Ramirez, Z Chen JoVE (Journal of Visualized Experiments), e51202, 2014 | 4 | 2014 |
H2-driven reduction of flavin by hydrogenase enables cleaner operation of nitroreductases for nitro-group to amine reductions MA Ramirez, S Joseph Srinivasan, SE Cleary, PMT Todd, HA Reeve, ... Frontiers in Catalysis 2, 906694, 2022 | 3 | 2022 |
Split-Intein Triggered Protein Hydrogels MA Ramirez, Z Chen Split Inteins: Methods and Protocols, 161-171, 2017 | 1 | 2017 |
Whither life? Conjectures on the future evolution of biochemistry JL Brewster, TJ Finn, MA Ramirez, WM Patrick Biology Letters 12 (8), 20160269, 2016 | 1 | 2016 |
Biocatalytic reductive amination as a route to isotopically labelled amino acids suitable for analysis of large proteins by NMR JS Rowbotham, JH Nicholson, MA Ramirez, K Urata, PMT Todd, ... Chemical Science 14 (43), 12160-12165, 2023 | | 2023 |
Combined biocatalytic reductive amination and deuteration to prepare isotopically labelled amino acids for NMR analysis of large proteins J Rowbotham, J Nicholson, M Ramirez, K Urata, P Todd, G Karunanithy, ... | | 2022 |
E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme Ene-reductases. SE Cleary, MA Ramirez, HA Reeve, CE Paul, KA Vincent Angewandte Chemie (Weinheim an der Bergstrasse, Germany) 133 (25), 13943-13947, 2021 | | 2021 |