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Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
B Hellenkamp, S Schmid, O Doroshenko, O Opanasyuk, R Kühnemuth, ...
Nature methods 15 (9), 669-676, 2018
4192018
Plasticity of an ultrafast interaction between nucleoporins and nuclear transport receptors
S Milles, D Mercadante, IV Aramburu, MR Jensen, N Banterle, C Koehler, ...
Cell 163 (3), 734-745, 2015
2822015
Predicting age from the transcriptome of human dermal fibroblasts
JG Fleischer, R Schulte, HH Tsai, S Tyagi, A Ibarra, MN Shokhirev, ...
Genome biology 19, 1-8, 2018
1672018
Nucleoporin-mediated regulation of cell identity genes
A Ibarra, C Benner, S Tyagi, J Cool, MW Hetzer
Genes & development 30 (20), 2253-2258, 2016
1642016
Click strategies for single-molecule protein fluorescence
S Milles, S Tyagi, N Banterle, C Koehler, V VanDelinder, T Plass, AP Neal, ...
Journal of the American Chemical Society 134 (11), 5187-5195, 2012
1372012
Age mosaicism across multiple scales in adult tissues
RA e Drigo, V Lev-Ram, S Tyagi, R Ramachandra, T Deerinck, ...
Cell metabolism 30 (2), 343-351. e3, 2019
1062019
Orthogonal spin labeling using click chemistry for in vitro and in vivo applications
S Kucher, S Korneev, S Tyagi, R Apfelbaum, D Grohmann, EA Lemke, ...
Journal of Magnetic Resonance 275, 38-45, 2017
762017
Continuous throughput and long-term observation of single-molecule FRET without immobilization
S Tyagi, V VanDelinder, N Banterle, G Fuertes, S Milles, M Agez, ...
Nature methods 11 (3), 297-300, 2014
582014
Genetically encoded click chemistry for single-molecule FRET of proteins
S Tyagi, EA Lemke
Methods in Cell Biology 113, 169-187, 2013
502013
Two differential binding mechanisms of FG-nucleoporins and nuclear transport receptors
PS Tan, IV Aramburu, D Mercadante, S Tyagi, A Chowdhury, D Spitz, ...
Cell reports 22 (13), 3660-3671, 2018
442018
Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids
S Tyagi, EA Lemke
Current Opinion in Structural Biology 32, 66-73, 2015
352015
Palladium-unleashed proteins: gentle aldehyde decaging for site-selective protein modification
RL Brabham, RJ Spears, J Walton, S Tyagi, EA Lemke, MA Fascione
Chemical communications 54 (12), 1501-1504, 2018
232018
Aging of human endocrine pancreatic cell types is heterogeneous and sex-specific
R Arrojo e Drigo, G Erikson, S Tyagi, J Capitanio, J Lyon, AF Spigelman, ...
Biorxiv, 729541, 2019
122019
Plasticity of Nucleoporin Nuclear Transport Receptor Interactions-Molecular Description of a Highly Dynamic, Ultrafast Interaction Mechanism
IV Aramburu, D Mercadante, S Milles, M Ringkjøbing, N Banterle, ...
Biophysical Journal 110 (3), 357a, 2016
12016
A new method to study the dynamics of genome architecture at nuclear pore complexes during cell differentiation.
S Tyagi, MW Hetzer
MOLECULAR BIOLOGY OF THE CELL 29 (26), 2018
2018
Longevity of Beta Cells and Protein Complexes Revealed by Isotope Imaging
E DRIGO, R ARROJO, BH TOYAMA, S TYAGI, V LEV-RAM, ...
Diabetes 67 (Supplement_1), 2018
2018
Dissecting Conformational Features and Binding Mechanisms of Phenylalanine-Glycine Rich Nucleoporins
PS Tan, IV Aramburu, S Tyagi, A Chowdhury, EA Lemke
Biophysical Journal 112 (3), 511a, 2017
2017
Plasticity of nucleoporin nuclear transport receptor interactions.
IV Aramburu, PS Tan, S Tyagi, E Lemke
MOLECULAR BIOLOGY OF THE CELL 27, 2016
2016
Conformational Dynamics of Molecular Chaperones Investigated by Single Molecule Multicolor Förster Resonance Energy Transfer
LV von Voithenberg, A Barth, S Tyagi, C Koehler, EA Lemke, DC Lamb
Biophysical Journal 108 (2), 501a-502a, 2015
2015
Continuous throughput and long-term observation of single-molecule FRET without immobilization for studying protein dynamics
S Tyagi, V Vandelinder, N Banterle, G Fuertes, S Milles, M Agez, ...
FEBS JOURNAL 281, 27-28, 2014
2014
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