A new subfamily of polyphosphate kinase 2 (class III PPK2) catalyzes both nucleoside monophosphate phosphorylation and nucleoside diphosphate phosphorylation K Motomura, R Hirota, M Okada, T Ikeda, T Ishida, A Kuroda Applied and environmental microbiology 80 (8), 2602-2608, 2014 | 111 | 2014 |
Oriented immobilization of antibodies on a silicon wafer using Si-tagged protein A T Ikeda, Y Hata, K Ninomiya, Y Ikura, K Takeguchi, S Aoyagi, R Hirota, ... Analytical Biochemistry 385 (1), 132-137, 2009 | 101 | 2009 |
Detection of antibody-antigen reaction by silicon nitride slot-ring biosensors using protein G T Taniguchi, A Hirowatari, T Ikeda, M Fukuyama, Y Amemiya, A Kuroda, ... Optics Communications 365, 16-23, 2016 | 73 | 2016 |
Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner T Ikeda, K Ninomiya, R Hirota, A Kuroda Protein expression and purification 71 (1), 91-95, 2010 | 68 | 2010 |
Why does the silica-binding protein “Si-tag” bind strongly to silica surfaces? Implications of conformational adaptation of the intrinsically disordered polypeptide to solid … T Ikeda, A Kuroda Colloids and Surfaces B: Biointerfaces 86 (2), 359-363, 2011 | 58 | 2011 |
A novel biocontainment strategy makes bacterial growth and survival dependent on phosphite R Hirota, K Abe, Z Katsuura, R Noguchi, S Moribe, K Motomura, T Ishida, ... Scientific reports 7, 44748, 2017 | 49 | 2017 |
Synthetic phosphorus metabolic pathway for biosafety and contamination management of cyanobacterial cultivation K Motomura, K Sano, S Watanabe, A Kanbara, AH Gamal Nasser, T Ikeda, ... ACS Synthetic Biology 7 (9), 2189-2198, 2018 | 48 | 2018 |
Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts K Kanda, T Ishida, R Hirota, S Ono, K Motomura, T Ikeda, K Kitamura, ... Journal of biotechnology 182, 68-73, 2014 | 46 | 2014 |
The silicon layer supports acid resistance of Bacillus cereus spores R Hirota, Y Hata, T Ikeda, T Ishida, A Kuroda Journal of bacteriology 192 (1), 111-116, 2010 | 45 | 2010 |
Affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag MAA Abdelhamid, K Motomura, T Ikeda, T Ishida, R Hirota, A Kuroda Applied microbiology and biotechnology 98 (12), 5677-5684, 2014 | 44 | 2014 |
Anabolic five subunit-type pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus TK-6 T Ikeda, T Ochiai, S Morita, A Nishiyama, E Yamada, H Arai, M Ishii, ... Biochemical and biophysical research communications 340 (1), 76-82, 2006 | 43 | 2006 |
Overproduction of YjbB reduces the level of polyphosphate in Escherichia coli: a hypothetical role of YjbB in phosphate export and polyphosphate accumulation K Motomura, R Hirota, N Ohnaka, M Okada, T Ikeda, T Morohoshi, ... FEMS microbiology letters 320 (1), 25-32, 2011 | 42 | 2011 |
Carboxylation reaction catalyzed by 2-oxoglutarate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus M Yamamoto, T Ikeda, H Arai, M Ishii, Y Igarashi Extremophiles 14 (1), 79-85, 2010 | 42 | 2010 |
Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506 R Hirota, S Yamane, T Fujibuchi, K Motomura, T Ishida, T Ikeda, A Kuroda Journal of bioscience and bioengineering 113 (4), 445-450, 2012 | 37 | 2012 |
Selective detection of antigen-antibody reaction using Si ring optical resonators M Fukuyama, S Yamatogi, H Ding, M Nishida, C Kawamoto, Y Amemiya, ... Japanese Journal of Applied Physics 49 (4S), 04DL09, 2010 | 37 | 2010 |
A novel ferredoxin-dependent glutamate synthase from the hydrogen-oxidizing chemoautotrophic bacterium Hydrogenobacter thermophilus TK-6 M Kameya, T Ikeda, M Nakamura, H Arai, M Ishii, Y Igarashi Journal of bacteriology 189 (7), 2805-2812, 2007 | 32 | 2007 |
The silica-binding Si-tag functions as an affinity tag even under denaturing conditions T Ikeda, K Motomura, Y Agou, T Ishida, R Hirota, A Kuroda Protein Expression and Purification 77 (2), 173-177, 2011 | 31 | 2011 |
Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy T Ishida, N Fujihara, T Nishimura, H Funabashi, R Hirota, T Ikeda, ... Genes and Environment 41 (1), 1-11, 2019 | 28 | 2019 |
Application of volcanic ash particles for protein affinity purification with a minimized silica-binding tag MAA Abdelhamid, T Ikeda, K Motomura, T Tanaka, T Ishida, R Hirota, ... Journal of bioscience and bioengineering 122 (5), 633-638, 2016 | 28 | 2016 |
Si ring optical resonators for integrated on-chip biosensing S Yamatogi, Y Amemiya, T Ikeda, A Kuroda, S Yokoyama Japanese Journal of Applied Physics 48 (4S), 04C188, 2009 | 26 | 2009 |