Michael W. Pfaffl
Michael W. Pfaffl
Professor of Molecular Physiology, TUM, Technische Universität München, Technical University of
Verified email at gene-quantification.info - Homepage
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Year
A new mathematical model for relative quantification in real-time RT–PCR
MW Pfaffl
Nucleic Acids Research 29 (9), e45-e45, 2001
268962001
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
SA Bustin, V Benes, JA Garson, J Hellemans, J Huggett, M Kubista, ...
Clinical Chemistry 55 (4), 611-622, 2009
95092009
Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
MW Pfaffl, GW Horgan, L Dempfle
Nucleic Acids Research 30 (9), e36-e36, 2002
68282002
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper–Excel-based tool using pair-wise correlations
MW Pfaffl, A Tichopad, C Prgomet, TP Neuvians
Biotechnology Letters 26 (6), 509-515, 2004
35082004
RNA integrity and the effect on the real-time qRT-PCR performance
S Fleige, MW Pfaffl
Molecular aspects of medicine 27 (2-3), 126-139, 2006
13972006
Quantitative real-time RT-PCR – a perspective
SA Bustin, V Benes, T Nolan, MW Pfaffl
Journal of molecular endocrinology 34 (3), 597-601, 2005
13792005
Chapter 3: Quantification strategies in real-time PCR
MW Pfaffl
A-Z of Quantitative PCR 1 (Chapter 3), 89-113, 2004
919*2004
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the …
C Théry, KW Witwer, E Aikawa, MJ Alcaraz, JD Anderson, ...
Journal of extracellular vesicles 7 (1), 1535750, 2018
7112018
Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR
S Fleige, V Walf, S Huch, C Prgomet, J Sehm, MW Pfaffl
Biotechnology Letters 28 (19), 1601-1613, 2006
5602006
Standardized determination of real‐time PCR efficiency from a single reaction set‐up
A Tichopad, M Dilger, G Schwarz, MW Pfaffl
Nucleic acids research 31 (20), e122-e122, 2003
5342003
The digital MIQE guidelines: minimum information for publication of quantitative digital PCR experiments
JF Huggett, CA Foy, V Benes, K Emslie, JA Garson, R Haynes, ...
Clinical Chemistry 59 (6), 892-902, 2013
4432013
Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR
MW Pfaffl, M Hageleit
Biotechnology Letters 23 (4), 275-282, 2001
3152001
Relative Quantification
MW Pfaffl
Real-time PCR 63, 63-82, 2006
2632006
RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization
T Hruz, M Wyss, M Docquier, MW Pfaffl, S Masanetz, L Borghi, ...
BMC Genomics 12 (1), 156, 2011
2522011
mRNA and microRNA quality control for RT-qPCR analysis
C Becker, A Hammerle-Fickinger, I Riedmaier, MW Pfaffl
Methods 50 (4), 237-243, 2010
2462010
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research
J Van Deun, P Mestdagh, P Agostinis, Ö Akay, S Anand, J Anckaert, ...
Nature Methods 14 (3), 228-232, 2017
2452017
Comparison of reverse transcriptases in gene expression analysis
A Ståhlberg, M Kubista, M Pfaffl
Clinical Chemistry 50 (9), 1678-1680, 2004
2442004
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper
B Mateescu, EJK Kowal, BWM van Balkom, S Bartel, SN Bhattacharyya, ...
Journal of extracellular vesicles 6 (1), 1286095, 2017
2432017
Normalization strategies for microRNA profiling experiments: a ‘normal’way to a hidden layer of complexity?
SU Meyer, MW Pfaffl, SE Ulbrich
Biotechnology Letters 32 (12), 1777-1788, 2010
2142010
The need for transparency and good practices in the qPCR literature
SA Bustin, V Benes, J Garson, J Hellemans, J Huggett, M Kubista, ...
Nature Methods 10 (11), 1063-1067, 2013
2102013
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