Michael W. Pfaffl

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	All	Since 2016
Citations	68051	31236
h-index	56	36
i10-index	154	103

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Co-authors

Mikael KubistaInstitute of Biotechnology, CAS, TATAA Biocenter AB, Life Genomics ABVerified email at tataa.com
Jo Vandesompeleprofessor in functional cancer genomics and applied bioinformaticsVerified email at ugent.be
Stephen BustinProfessor of Molecular Medicine, Anglia Ruskin UniversityVerified email at aru.ac.uk
Jim F HuggettLGC/University of SurreyVerified email at lgcgroup.com
Jeremy GarsonUCLVerified email at ucl.ac.uk
Jan HellemansBiogazelleVerified email at biogazelle.com
Carl WittwerProfessor of Pathology, University of UtahVerified email at path.utah.edu
Dominik BuschmannPostdoctoral Fellow at Johns Hopkins MedicineVerified email at wzw.tum.de
Bajram BerishaUniversity of PrishtinaVerified email at uni-pr.edu
Fred SinowatzProfessor of Anatomy, Histology and Embryology, University of MunichVerified email at anat.vetmed.uni-muenchen.de
Ivan Penchev GeorgievProfesor in Physiology, Trakia University, Stara Zagora, BulgariaVerified email at uni-sz.bg
Jean-Francois HOCQUETTEDirecteur de recherchesVerified email at inra.fr
Prof. Ivan Penchev GeorgievTrakia University, Fakulty of Veterinary MedicineVerified email at trakia-uni.bg
Stefaan DerveauxManaging Director WaferGen Biosystems Europe S.à r.l.Verified email at wafergen.com
Sebastian KaiserDepartment of Statistics, LMU MunichVerified email at stat.uni-muenchen.de
Veronika MussackVerified email at wzw.tum.de
Steffen SassHelmholtz Zentrum MünchenVerified email at helmholtz-muenchen.de
Philip ZimmermannCEO, Nebion AGVerified email at nebion.com
Phebe VerbruggheResearch Associate, University of Western AustraliaVerified email at uwa.edu.au
Lorenzo BorghiUniversity of ZurichVerified email at uzh.ch

Michael W. Pfaffl

Professor of Molecular Physiology, TUM, Technische Universität München, Technical University of

Verified email at gene-quantification.info - Homepage

Molecular Physiology qPCR Molecular Diagnostics Liquid Biopsy Transcriptomics


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A new mathematical model for relative quantification in real-time RT–PCR MW Pfaffl Nucleic Acids Research 29 (9), e45-e45, 2001	29234	2001
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments SA Bustin, V Benes, JA Garson, J Hellemans, J Huggett, M Kubista, ... Clinical Chemistry 55 (4), 611-622, 2009	10912	2009
Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR MW Pfaffl, GW Horgan, L Dempfle Nucleic Acids Research 30 (9), e36-e36, 2002	7300	2002
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper–Excel-based tool using pair-wise correlations MW Pfaffl, A Tichopad, C Prgomet, TP Neuvians Biotechnology Letters 26 (6), 509-515, 2004	3950	2004
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the … C Théry, KW Witwer, E Aikawa, MJ Alcaraz, JD Anderson, ... Journal of extracellular vesicles 7 (1), 1535750, 2018	1825	2018
RNA integrity and the effect on the real-time qRT-PCR performance S Fleige, MW Pfaffl Molecular aspects of medicine 27 (2-3), 126-139, 2006	1526	2006
Quantitative real-time RT-PCR – a perspective SA Bustin, V Benes, T Nolan, MW Pfaffl Journal of molecular endocrinology 34 (3), 597-601, 2005	1461	2005
Chapter 3: Quantification strategies in real-time PCR MW Pfaffl A-Z of Quantitative PCR 1 (Chapter 3), 89-113, 2004	1032*	2004
Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR S Fleige, V Walf, S Huch, C Prgomet, J Sehm, MW Pfaffl Biotechnology Letters 28 (19), 1601-1613, 2006	607	2006
Standardized determination of real‐time PCR efficiency from a single reaction set‐up A Tichopad, M Dilger, G Schwarz, MW Pfaffl Nucleic acids research 31 (20), e122-e122, 2003	562	2003
The digital MIQE guidelines: minimum information for publication of quantitative digital PCR experiments JF Huggett, CA Foy, V Benes, K Emslie, JA Garson, R Haynes, ... Clinical Chemistry 59 (6), 892-902, 2013	557	2013
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research J Van Deun, P Mestdagh, P Agostinis, Ö Akay, S Anand, J Anckaert, ... Nature Methods 14 (3), 228-232, 2017	396	2017
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper B Mateescu, EJK Kowal, BWM van Balkom, S Bartel, SN Bhattacharyya, ... Journal of extracellular vesicles 6 (1), 1286095, 2017	344	2017
Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR MW Pfaffl, M Hageleit Biotechnology Letters 23 (4), 275-282, 2001	336	2001
Relative Quantification MW Pfaffl Real-time PCR 63, 63-82, 2006	319	2006
RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization T Hruz, M Wyss, M Docquier, MW Pfaffl, S Masanetz, L Borghi, ... BMC Genomics 12 (1), 156, 2011	271	2011
mRNA and microRNA quality control for RT-qPCR analysis C Becker, A Hammerle-Fickinger, I Riedmaier, MW Pfaffl Methods 50 (4), 237-243, 2010	270	2010
Comparison of reverse transcriptases in gene expression analysis A Ståhlberg, M Kubista, M Pfaffl Clinical Chemistry 50 (9), 1678-1680, 2004	259	2004
How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments D Svec, A Tichopad, V Novosadova, MW Pfaffl, M Kubista Biomolecular detection and quantification 3, 9-16, 2015	255	2015
The need for transparency and good practices in the qPCR literature SA Bustin, V Benes, J Garson, J Hellemans, J Huggett, M Kubista, ... Nature Methods 10 (11), 1063-1067, 2013	231	2013

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