| A new mathematical model for relative quantification in real-time RT–PCR MW Pfaffl Nucleic Acids Research 29 (9), e45-e45, 2001 | 32737 | 2001 |
| The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments SA Bustin, V Benes, JA Garson, J Hellemans, J Huggett, M Kubista, ... Clinical Chemistry 55 (4), 611-622, 2009 | 13178 | 2009 |
| Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR MW Pfaffl, GW Horgan, L Dempfle Nucleic Acids Research 30 (9), e36-e36, 2002 | 7911 | 2002 |
| Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper–Excel-based tool using pair-wise correlations MW Pfaffl, A Tichopad, C Prgomet, TP Neuvians Biotechnology Letters 26 (6), 509-515, 2004 | 4597 | 2004 |
| Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the … C Théry, KW Witwer, E Aikawa, MJ Alcaraz, JD Anderson, ... Journal of Extracellular Vesicles 7 (1), 1535750, 2018 | 4466 | 2018 |
| RNA integrity and the effect on the real-time qRT-PCR performance S Fleige, MW Pfaffl Molecular Aspects of Medicine 27 (2-3), 126-139, 2006 | 1707 | 2006 |
| Quantitative real-time RT-PCR – a perspective. SA Bustin, V Benes, T Nolan, MW Pfaffl Journal of Molecular Endocrinology 34 (3), 597-601, 2005 | 1659 | 2005 |
| Quantification strategies in real-time PCR MW Pfaffl A-Z of Quantitative PCR edited by SA Bustin, 89-113, 2004 | 1229* | 2004 |
| The digital MIQE guidelines: minimum information for publication of quantitative digital PCR experiments JF Huggett, CA Foy, V Benes, K Emslie, JA Garson, R Haynes, ... Clinical Chemistry 59 (6), 892-902, 2013 | 712 | 2013 |
| Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR S Fleige, V Walf, S Huch, C Prgomet, J Sehm, MW Pfaffl Biotechnology Letters 28 (19), 1601-1613, 2006 | 676 | 2006 |
| EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research J Van Deun, P Mestdagh, P Agostinis, Ö Akay, S Anand, J Anckaert, ... Nature Methods 14 (3), 228-232, 2017 | 629 | 2017 |
| Standardized determination of real‐time PCR efficiency from a single reaction set‐up A Tichopad, M Dilger, G Schwarz, MW Pfaffl Nucleic Acids Research 31 (20), e122-e122, 2003 | 605 | 2003 |
| Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper B Mateescu, EJK Kowal, BWM van Balkom, S Bartel, SN Bhattacharyya, ... Journal of Extracellular Vesicles 6 (1), 1286095, 2017 | 524 | 2017 |
| How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments D Svec, A Tichopad, V Novosadova, MW Pfaffl, M Kubista Biomolecular Detection and Quantification 3, 9-16, 2015 | 399 | 2015 |
| Relative Quantification MW Pfaffl „Real-time PCR“ edited by M. Tevfik Dorak, 63-82, 2006 | 388 | 2006 |
| Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR MW Pfaffl, M Hageleit Biotechnology Letters 23 (4), 275-282, 2001 | 368 | 2001 |
| mRNA and microRNA quality control for RT-qPCR analysis C Becker, A Hammerle-Fickinger, I Riedmaier, MW Pfaffl Methods 50 (4), 237-243, 2010 | 316 | 2010 |
| RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization T Hruz, M Wyss, M Docquier, MW Pfaffl, S Masanetz, L Borghi, ... BMC Genomics 12 (1), 156, 2011 | 309 | 2011 |
| Comparison of reverse transcriptases in gene expression analysis A Ståhlberg, M Kubista, M Pfaffl Clinical Chemistry 50 (9), 1678-1680, 2004 | 275 | 2004 |
| The need for transparency and good practices in the qPCR literature SA Bustin, V Benes, J Garson, J Hellemans, J Huggett, M Kubista, ... Nature Methods 10 (11), 1063-1067, 2013 | 273 | 2013 |
Mikael KubistaInstitute of Biotechnology, CAS, TATAA Biocenter AB, Life Genomics ABVerified email at tataa.com
