Kentaro Miyazaki
Zitiert von
Zitiert von
How enzymes adapt: lessons from directed evolution
FH Arnold, PL Wintrode, K Miyazaki, A Gershenson
Trends in biochemical sciences 26 (2), 100-106, 2001
Directed evolution study of temperature adaptation in a psychrophilic enzyme
K Miyazaki, PL Wintrode, RA Grayling, DN Rubingh, FH Arnold
Journal of molecular biology 297 (4), 1015-1026, 2000
Functional metagenomics for enzyme discovery: challenges to efficient screening
T Uchiyama, K Miyazaki
Current Opinion in Biotechnology 20 (6), 616-622, 2009
Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function
K Miyazaki, FH Arnold
Journal of molecular evolution 49 (6), 716-720, 1999
A hyperthermophilic laccase from Thermus thermophilus HB27
K Miyazaki
Extremophiles 9 (6), 415-425, 2005
Creating random mutagenesis libraries using megaprimer PCR of whole plasmid
K Miyazaki, M Takenouchi
Biotechniques 33 (5), 1033-1038, 2002
Functional screening of a metagenomic library for genes involved in microbial degradation of aromatic compounds
H Suenaga, T Ohnuki, K Miyazaki
Environmental Microbiology 9 (9), 2289-2297, 2007
Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution
PL Wintrode, K Miyazaki, FH Arnold
Journal of biological chemistry 275 (41), 31635-31640, 2000
Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution
K Miyazaki, M Takenouchi, H Kondo, N Noro, M Suzuki, S Tsuda
Journal of Biological Chemistry 281 (15), 10236-10242, 2006
MEGAWHOP cloning: a method of creating random mutagenesis libraries via megaprimer PCR of whole plasmids
K Miyazaki
Methods in enzymology 498, 399-406, 2011
Product-induced gene expression, a product-responsive reporter assay used to screen metagenomic libraries for enzyme-encoding genes
T Uchiyama, K Miyazaki
Applied and environmental microbiology 76 (21), 7029-7035, 2010
Purification, catalytic properties, and thermal stability of threo-Ds-3-isopropylmalate dehydrogenase coded by leuB gene from an extreme thermophile, Thermus thermophilus …
T Yamada, N Akutsu, K Miyazaki, K Kakinuma, M Yoshida, T Oshima
The Journal of Biochemistry 108 (3), 449-456, 1990
Characterization of a novel β-glucosidase from a compost microbial metagenome with strong transglycosylation activity
T Uchiyama, K Miyazaki, K Yaoi
Journal of Biological Chemistry 288 (25), 18325-18334, 2013
Creating random mutagenesis libraries by megaprimer PCR of whole plasmid (MEGAWHOP)
K Miyazaki
Directed Evolution Library Creation, 23-28, 2003
Metagenomic screening for bleomycin resistance genes
T Mori, S Mizuta, H Suenaga, K Miyazaki
Applied and environmental microbiology 74 (21), 6803-6805, 2008
Novel organization of aromatic degradation pathway genes in a microbial community as revealed by metagenomic analysis
H Suenaga, Y Koyama, M Miyakoshi, R Miyazaki, H Yano, M Sota, ...
The ISME journal 3 (12), 1335-1348, 2009
Cloning, expression and characteristics of a novel alkalistable and thermostable xylanase encoding gene (Mxyl) retrieved from compost-soil metagenome
D Verma, Y Kawarabayasi, K Miyazaki, T Satyanarayana
PLoS one 8 (1), e52459, 2013
Patterns of adaptation in a laboratory evolved thermophilic enzyme
PL Wintrode, K Miyazaki, FH Arnold
Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular …, 2001
X-ray structure of a two-domain type laccase: a missing link in the evolution of multi-copper proteins
H Komori, K Miyazaki, Y Higuchi
FEBS letters 583 (7), 1189-1195, 2009
Bacterial cellular engineering by genome editing and gene silencing
N Nakashima, K Miyazaki
International journal of molecular sciences 15 (2), 2773-2793, 2014
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